Small‐Angle X‐Ray Scattering and Structural Biology

APR 01, 1985

Intense x‐ray synchrotron radiation, from multi‐GeV electron storage rings, is giving new life to an old technique for studying the structure of the large biological molecules that control the shape of the cell.

DOI: 10.1063/1.880970

Sebastian Doniach

It has been 25 years since Max Perutz and John Kendrew, working in Cambridge, England, used x‐ray crystallography to determine in three dimensions the molecular structure of the oxygen‐transport proteins hemoglobin and myoglobin. Their technique—xray diffraction from crystallized samples—continues to be one of the central sources of structural information for biology at the molecular level. Now, however, the advent of powerful new synchrotron x‐ray sources based on multi‐GeV electron storage rings is opening up new ways in which one can probe biological structure at the subcellular level.

References

1. A. Gabriel, Rev. Sci. Instrum. 48, 1303 (1977).https://doi.org/RSINAK
2. A. R. Faruqui, H. E. Huxley, Ann. Rev. Biophys. Bioeng. 12, 381 (1983).
3. P. Dustin, Sci. Am., August 1980, p. 66.
4. E. M. Mandelkow, A. Harmsen, E. Mandelkow, J. Bordas, Nature 287, 595 (1980).https://doi.org/NATUAS
5. R. K. Clayton, W. R. Sistrom, eds., The Photosynthetic Bacteria, Plenum, New York (1978).
6. G. Feher, M. Y. Okamura in ref. 5, p. 349.
7. W. W. Parson, Ann. Rev. Biophys. Bioeng. 11, 57 (1982).
8. J. K. Blasie, J. M. Pachence, A. Tavormina, P. L. Dutton, J. Stamatoff, P. Eisenberger, G. Brown, Biochim. Biophys. Acta 723, 350 (1983).https://doi.org/BBACAQ
9. H. Michel, J. Mol. Biol. 158, 567 (1982).https://doi.org/JMOBAK
10. M. F. Moody, P. Vachette, A. M. Foote, A. Tardieu, M. H. J. Koch, J. Bordas, Proc. Natl. Acad. Sci. USA 77, 4040 (1980).https://doi.org/PNASA6
11. For a review, see H. B. Stuhrmann, Q. Rev. Biophys. 14, no. 3, 433 (1981).https://doi.org/QURBAW
12. H. B. Stuhrmann, H. Notbohm, Proc. Natl. Acad. Sci. USA 78, 6216 (1981).https://doi.org/PNASA6
13. R. C. Miake‐Lye, S. Doniach, K. O. Hodgson, Biophys. J. 41, 287 (1983).https://doi.org/BIOJAU
14. M. Seul, P. Eisenberger, H. M. McConnell, Proc. Natl. Acad. Sci. USA 80, 5795 (1983).https://doi.org/PNASA6
15. R. Kornberg, E. Uzgiris, Nature 301, 125 (1982).https://doi.org/NATUAS

More about the Authors

Sebastian Doniach. Stanford University.