New microscopy technique uses structured illumination
DOI: 10.1063/PT.5.029161
MIT Technology Review : Because the macromolecular dynamics of living cells take place so quickly and on such a small scale, tracking them requires very specialized microscopy techniques. Although high-resolution microscopy can capture images of cells by tagging them with fluorescent molecules, the intense light required can damage the cells. Researchers have now found a way to image cells far faster, with much less light, and at higher resolutions by using a new technique called structured illumination microscopy. In their paper published today in Science, the researchers describe in detail the two approaches they used to achieve live-cell imaging at resolutions finer than 100 nm.